Dear All,

There are two recently published papers that bring a sobering perspective on the potential pitfalls and potentially problematic data published from qPCR. The first published in Nature Methods surveyed 1700 articles using qPCR with the principal conclusions that inadequate technical detail is provided to assess data quality with RNA integrity and reference gene normalization highlighted as inadequate ( http://www.nature.com/nmeth/journal/v10/n11/full/nmeth.2697.html ). The second published in Molecular Oncology surveyed 179 colorectal cancer publications for adequate normalization of qPCR data showing just three percent could be adequately assessed with common errors described

http://www.moloncol.org/article/S1574-7891(13)00188-9/

Producing high quality data from qPCR experiments is a simple matter of following a well defined procedure with some initial validation processes to assure solid results. Our recently published article entitled “The State of RT-Quantitative PCR: Firsthand Observations of Implementation of Minimum Information for the Publication of Quantitative Real-Time PCR Experiments” in the Journal of Molecular Microbiology and Biotechnology nicely summarizes this entire process in about five and a half pages. Based on the high demand for reprints, the Bio-Rad global marketing has generously purchased the Open Access licence so the paper can now be downloaded free-of-charge from the journal web site  ( http://www.karger.com/Article/PDF/356189 ). We have also recorded an accompanying, 24-minute video to guide you through the entire q-PCR process:

We continue to make every effort to summarize qPCR experimental design to help assure that you generate high quality data in a very short time frame and hope you find these tools useful. As always, your support and commitment to Bio-Rad help us in our ongoing efforts to bring you the tools and training to maximize success in your research. Feel free to contact your local sales team (cc’d on this message) to learn about our great solutions for your projects. Have a great start to the year and stay warm!!

Best,

Sean Taylor
Field Application Specialist
E:     sean_taylor@bio-rad.com

For All Technical Support
T:    1-800-424-6723
E:    tech_canada@bio-rad.com

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