Which Detergent Lysis Buffer Should You Use?

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https://advansta.com/lysis-buffers/

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How to Write a Great Research Paper

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Feed Navigator

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https://www.terkko.helsinki.fi/feednavigator/?journal=Virology&c=immunology+and+microbiology&c2=Journals#

Subcellular localization of proteins

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Subcellular localization of Eukaryotic proteins

http://www.imtech.res.in/raghava/eslpred/

Protein Localization Database for Human and Arabidopsis

https://www.rostlab.org/services/locDB/

Reverse translation of amino acid sequences based on frequency of amino acid codons in organism; optimize DNA or Protein sequence

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“Since there are only 20 genetically encoded amino acids, most amino acids are coded for by multiple codons. Bacteria and mammals prefer to use different codons, so that mammalian genes frequently use codons which are rarely employed in bacteria and vice versa. So it can happen that a mammalian gene may be expressed very poorly in bacteria, a significant problem. A way to deal with this is to look at the mammalian sequence, figure out which codons are optimal for bacterial expression, and synthesize an appropropriate DNA sequence specifically to efficiently express the mammalian gene in bacteria.” from  http://www.encorbio.com/protocols/Codon.htm

http://www.molbiol.ru/eng/scripts/01_19.html

http://www.zbio.net/eng/scripts/01_19.html

http://www.encorbio.com/protocols/Codon.htm

http://www.kazusa.or.jp/codon/

http://www.geneinfinity.org/sp/sp_codonusage.html

http://genomes.urv.es/OPTIMIZER/

http://www.jcat.de/

https://www.genscript.com/tools/rare-codon-analysis

https://www.biologicscorp.com/tools/CAICalculator/

https://omictools.com/optimizer-tool

https://bitesizebio.com/36561/codon-optimization-101/

 

Cell-PLoc: A package of web-servers for predicting subcellular localization of proteins in different organisms

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http://www.csbio.sjtu.edu.cn/bioinf/Cell-PLoc/

Short linear motif Scanner

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1.Eukaryotic Linear Motif resource

http://elm.eu.org/

https://en.wikipedia.org/wiki/Eukaryotic_Linear_Motif_resource

2. Minimotif Miner

http://mnm.engr.uconn.edu

http://minimotifminer.org

https://en.wikipedia.org/wiki/Minimotif_Miner

3. Phospho.ELM

http://phospho.elm.eu.org/

https://en.wikipedia.org/wiki/Phospho.ELM

4.  Post-translational modifications (PTMs) of  CUCKOO Workgroup 

http://www.biocuckoo.org/

http://cplm.biocuckoo.org/index.php

5. List of biological databases

https://en.wikipedia.org/wiki/List_of_biological_databases

https://en.wikipedia.org/wiki/Short_linear_motif

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