2X Rapid Ligation Buffer:

132 mM Tris-HCl, 20 mM MgCl2, 2 mM DTT, 2 mM ATP, 15% PEG 6000, pH 7.6

 

For ligation of long DNA fragments, prepare the buffer below:

10X T4 DNA Ligase Buffer:

500 mM Tris-HCl, 100 mM MgCl2, 50 mM DTT, 10 mM ATP, pH 7.6

From: T4 DNA ligase Rapid

Then, follow the instruction in “TaKaRa Clontech DNA Ligation Kit for Long Fragments”

TaKaRa Clontech DNA Ligation Kit for Long Fragments

 

 

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