One pair of primers with several PCR product sizes

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One gene and several size PCR products

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Measuring the Formaldehyde Protein–DNA Cross-Link Reversal Rate

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http://pubs.acs.org/doi/abs/10.1021/ac501354y

ac-2014-01354y_0002

From Sequence to Structure

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From Sequence to Structure

http://www3.uah.es/farmamol/New_Science_Press/nsp-protein-1.pdf

http://www3.uah.es/farmamol/

http://www3.uah.es/farmamol/New_Science_Press/nsp-protein-1.pdf

Transcription Factors

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1,672 Transcription Factors:

Browse Transcription Factors hg19 – resource_browser-2

From: http://fantom.gsc.riken.jp/5/sstar/Browse_Transcription_Factors_hg19http://fantom.gsc.riken.jp/5/sstar/Browse_Transcription_Factors_hg19

 

1544 Transcription Factors:

http://www.bioguo.org/AnimalTFDB/BrowseAllTF.php?spe=Homo_sapiens

Rosetta: determine the 3-dimensional shapes of proteins

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http://boinc.bakerlab.org/rosetta/

FANTOM5 SSTAR (Semantic catalog of Samples, Transcription initiation And Regulators)

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http://fantom.gsc.riken.jp/5/sstar/Main_Page

dCas9-p300 CRISPR Gene Activator

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Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300. This approach has been independently validated by the Gersbach lab (Duke University) to activate genes at both proximal and distal locations relative the transcriptional start site (TSS). The dCas9-p300 histone acetylation approach represents a distinct mechanism of action relative to dCas9-VP64 or other similar gene activation motifs. While activation domains, such as VP64, help recruit transcription complexes to the promoter region, they are at the mercy of the epigenetic state of the gene and dependent on the availability of additional transcriptional proteins. Conversely, the p300 histone acetyltransferase protein opens a transcriptional highway by releasing the DNA from its heterochromatin state and allowing for continued and robust gene expression by the endogenous cellular machinery.

http://www.sigmaaldrich.com/technical-documents/articles/biology/dcas9-p300-crispr-gene-activator.printerview.html?utm_source=homepage-12-11-15&utm_medium=hero-banner-anonymous&utm_campaign=gene-activator

http://www.sigmaaldrich.com/technical-documents/articles/biology/dcas9-p300-crispr-gene-activator.html?utm_source=homepage-12-11-15&utm_medium=hero-banner-anonymous&utm_campaign=gene-activator#

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