I assume that you mean changing the amino acid sequence in a single protein in an organism to one that no longer reflects the sequence predicted from the DNA sequence. In that case, I would suggest using ADAR (Double-stranded RNA-specific adenosine deaminase) or similar enzymes. ADAR deaminates adenosine to inosine, which is read by the ribosome as a guanine. I know that it’s activity is regulated, but I’m not sure how you could redirect it (perhaps with an antisense RNA that yields a double-stranded binding stretch in the target RNA.
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answered May 27 at 16:44
Michael Rogers