ARB Project; rRNA phylogenetic treeing

Leave a comment

http://www.arb-home.de  (rRNA sequence data phylogenetic treeing for rRNA and protein)

https://www.arb-silva.de    (A comprehensive on-line resource for quality checked and aligned ribosomal RNA sequence data.)


16s sequencing:

Primers for microbial detection by sequencing.

Primer Sequence
For bacteria 10F 5′-GTTTGATCCTGGCTCA
800R 5′-TACCAGGGTATCTAATCC
For fungi ITS1F 5′-GTAACAAGGT(T/C)TCCGT
ITS1R 5′-CGTTCTTCATCGATG
For full-length sequencing(only for bacteria
27F 5′-AGAGTTTGATCCTGGCTCAG
1492R 5′-GGCTACCTTGTTACGACTT

From:

http://www.bexnet.co.jp/english/product/trust/gene-analysis/microbial.html

 

Advertisements

pPSU Plasmids for DNA Markers (Ladder)

Leave a comment

http://www.personal.psu.edu/sxt30/projects_DNA_ladders.html

https://www.addgene.org/89439

https://www.addgene.org/89566