GelRed & GelGreen – DNA Stains

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It is 10,000 x.

Buy 500 ul for 105 $.

Dilute it by water to 10x, that is, add 500 ml water to 500 ul.

Instead of using it as Precast or Post-staining protocols, mix 1 ul of GelRed with 5 ul of DNA and run it.

The Online Bioinformatics Resources Collection (OBRC)

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The Online Bioinformatics Resources Collection (OBRC) contains annotations and links for 2458 bioinformatics databases and software tools.

T4 DNA ligase recipe

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 2X Rapid Ligation Buffer:

132 mM Tris-HCl, 20 mM MgCl2, 2 mM DTT, 2 mM ATP, 15% PEG 6000, pH 7.6


For ligation of long DNA fragments, prepare the buffer below:

10X T4 DNA Ligase Buffer:

500 mM Tris-HCl, 100 mM MgCl2, 50 mM DTT, 10 mM ATP, pH 7.6

From: T4 DNA ligase Rapid

Then, follow the instruction in “TaKaRa Clontech DNA Ligation Kit for Long Fragments”

TaKaRa Clontech DNA Ligation Kit for Long Fragments



Rubidium Chloride Competent Cells Protocol

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Which Detergent Lysis Buffer Should You Use?

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How to Write a Great Research Paper

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